TARGET AUDIENCE The course is targeted at tissue culturists, breeders, physiologists, and agronomists working on varietal improvement using novel methods such as genetic engineering in the public, private, and NGO sectors. INTRODUCTION Rice transformation in Indica rice and elite mega varieties offers enormous opportunities in rice improvement by allowing the introduction of novel genes, which may not be possible through conventional breeding. It is an important tool for rice functional genomic studies. Most rice transformation laboratories use the more amenable japonica cultivars in their research; only a few, including the Genetic Transformation Laboratory (GTL) at IRRI, have developed routine protocols for high-efficiency and reproducible transformation of recalcitrant Indica varieties. GTL routinely produces 6,000 events annually with a transformation efficiency of 30–50%, depending on the promoter and gene combination. A routine co-transformation efficiency of 10–15% is achieved for selectable marker-free transformation. A quick, reliable, low cost molecular assay for the presence of the transgene and copy number was established at IRRI, as well as the capacity to select homozygous plants at the T1 generation. OBJECTIVES This training course aims to
1. Transformation
3. Molecular analysis of regenerants
5. Designing primers for zygosity and event--specific screening TRAINING STAFF IRRI staff from GTL, PBGB, CESD, and C4 Rice Center involved in research, product development, and application of genetic transformation will be invited as resource persons and lab trainers. DATE: POSTPONED VENUE Genetic Transformation Laboratory and Molecular Marker Applications Lecture Room Plant Breeding, Genetics and Biotechnology Division International Rice Research Institute (IRRI) Los Baños, Laguna, Philippines CONTACT INFORMATION Dr. Inez Slamet-Loedin Senior Scientist, Genetic Transformation Laboratory, IRRI Dr. Noel Magor Head, Training Center, IRRI International Rice Research Institute (IRRI) DAPO Box 7777, Metro Manila, Philippines Tel no. (63-2) 580-5600 E-mail: IRRITraining@irri.org Fax: (63-2) 580-5699; 891-1292; 845-0606 |
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